Bacteria

Home   /   Bacteria

Liberibacter’ spp. (Huanglongbing-HLB citrus disease). Rapid screening test®

Ref: HLB/100.

Complete real-time PCR kit® for direct screening for 100 tests.

Includes:

  • 2 vials complete Master mix (universal primers+Taqman FAM/TAMRA probe) for 50 reactions each
  • 2 vials containing 50 pieces of Whatman 3MM paper
  • 1 vial containing sterile distilled water DN/RNase-free for master mix preparation
  • 1 tube 12mL distilled water DN/RNase-free for sample preparation
  • 2 vials containing positive controls immobilized on paper
  • 2 vials containing healthy controls immobilized on paper
  • Detailed protocol.

Ref: HLBmix.

Complete Master mix® for 50 tests.

Ref:HLB-PC.

Positive control ‘Ca. Liberibacter’ (2 spots immobilized on paper).

Ref:HLB-HC.

Healthy citrus control (2 spots immobilized on paper).

A Hlb Cand. Liber. spp

‘Ca. Liberibacter’ ssp. (Huanglongbing-HLB citrus disease). Identification kit for species associated (‘Ca. L. asiaticus, africanus and/or americanus’).

Ref: HLB-ID/150.

Complete real-time PCR kit® for direct identification of species associated to HLB, for 150 tests (inquiries according the species).
Includes:

  • 1 package containing 2 paper membranes (7x13cm) (Whatman 3MM) for direct analysis of non-previously extracted or new samples if necessary.

  • 3 vials (blue cap) containing 1mL distilled sterile water DN/RNases free for master mix preparation.

  • 3 vials (yellow cap) containing lyophilized complete master mix (specific primers against ‘Ca. L. africanus, americanus, and asiaticus’ primers and TaqMan FAM/TAMRA probe) for specific detection-identification of any of the three ‘Ca. Liberibacter’ species associated to citrus HLB disease  (each tube contains enough for 50 reactions using 9 μL/reaction).

  • 3 vials (red cap) containing 1 immobilized non-virulent positive control spotted on a piece of membrane (‘Ca. Liberibacter africanus, americanus, and asiaticus’ in different vials).

  • 3 vials (green cap) containing 1 immobilized negative citrus control/vial spotted on a piece of membrane (3 μL per spot of crude extract from a certified sweet orange healthy plant).

  • 1 detailed protocol.

Ref: HLBmix-asiaticus; HLBmix-africanus; HLBmix-americanus.

Complete Master mix® specific to the species (according your in quiry) for 50 tests.
 Incluye: 

  • 1 vial of the specific Master mix complete according to the inquiry (specific primers+Taqman FAM/TAMRA probe) for 50 reactions.

Ref:HLB-PC asiaticus; HLB-PC africanus o HLB-PC americanus.

Positive non virulent control ‘Ca. Liberibacter’ spp. specific according the inquiry (2 spots immobilized on paper).
Incluye:

  • 2 vials containing specific positive control (according the inquiry) non virulent immobilized on paper.

Ref:HLB-HC.

Healthy negative citrus control certified free of pathogens, immobilized on paper.
Incluye:

  • 2 vials containing healthy extract of certified free of pathogens citrus, immobilized on paper. 

‘Candidatus Liberibacter solanacearum’ (Potato zebra chip and disorders in solanaceous and in other hosts)

Ref: CaLsol/100.

Complete real-time PCR kit® for 100 tests.

Includes:

  • 2 vials complete Master mix (universal primers+Taqman FAM/TAMRA probe) for 50 reactions each
  • 2 vials containing 50 pieces of Whatman 3MM paper
  • 1 vial containing sterile distilled water DN/RNase-free for master mix preparation
  • 1 tube 12mL distilled water DN/RNase-free for sample preparation
  • 2 vials containing positive controls immobilized on paper
  • 2 vials containing healthy controls immobilized on paper, vii) Detailed protocol.

Ref: CaLsolmix.

Complete Master mix® for 50 tests.

Ref: CaLsol-PC.

Positive control ‘Ca. Liberibacter solanacearum’ (2 spots on paper).

Ref: CaLsol-HC.

Healthy plant control (2 spots immobilized on paper).

A Cand. Liber. r solanc

Erwinia amylovora (Fire blight)

Ref: Ea-Enrich.

Enrichment DASI-ELISA® complete kit based on 5H+3B/IVIA specific monoclonal antibodies for 1000 tests.

Includes:

  • Polyclonal immunoglobulins 6089+EPS 1430/IVIA anti E. amylovora for coating
  • Positive non-virulent control
  • Negative control
  • Specific monoclonal antibodies 5H+3B/IVIA against E. amylovora
  • Anti-mouse immunoglobulins alkaline phosphatase conjugated
  • Selective medium for enrichment
  • Additives for extraction buffer
  • 10 ELISA plates
  • Detailed protocol.

Ref: MAb-Ea.

Specific monoclonal antibodies 5H+3B/IVIA, 1000 DASI-ELISA tests.

Ref: PAb-Ea.

Polyclonal antibodies 6089+EPS 1430/IVIA (0.5 ml crude antiserum).

Ref: IF-Ea.

Indirect immunofluorescence (specific monoclonal antibody 7A-IVIA), 0,5 ml.

Includes:

  • Specific monoclonal antibody 7A-IVIA, 0,5 ml
  • Antibodies anti-mouse (GAM) linked with FITC, 0,5 ml.

Ref: Ea-PC.

Positive non-virulent control for ELISA and/or Immunofluorescence.

Control positivo no virulento para ELISA o Inmunofluorescencia.Inmunofluorescencia indirecta

Pectobacterium atrosepticum (Potato black leg)

Ref: Pa-Enrich.

Enrichment DASI-ELISA® complete kit based on the specific monoclonal antibody 4G4-IVIA for 1000 tests.

Includes:

  • Polyclonal immunoglobulins anti P. atrosepticum for coating,
  • Positive non-virulent control,
  • Negative control,
  • Specific monoclonal antibody 4G4-IVIA against P. atrosepticum,
  • Anti-mouse immunoglobulins alkaline phosphatase conjugated,
  • Semi-selective medium for enrichment,
  • 10 ELISA plates,
  • Detailed protocol.

Ref: MAb4G4-Pa.

Specific monoclonal antibody 4G4-IVIA, 1000 DASI-ELISA tests.

Ref: IF-Pa.

Indirect immunofluorescence (specific monoclonal antibody 4G4-IVIA) 0,5 ml.

Includes:

  • Specific monoclonal antibody 4G4-IVIA, 0,5 ml,
  • Antibodies anti-mouse (GAM) linked with FITC, 0,5 ml.

Pseudomonas savastanoi pv. savastanoi (Olive knot)

Ref: Psavas.

Indirect-ELISA kit based on polyclonal antibodies for 1000 tests.

Includes:

  • Polyclonal immunoglobulins,
  • Positive control,
  • Negative control,
  • Anti-rabbit immunoglobulins alkaline phosphatase conjugated,
  • Detailed protocol.

Ralstonia solanacearum (Potato brown rot)

Ref: Rs-Enrich.

Enrichment DASI-ELISA® complete kit based on 8B-IVIA specific monoclonal antibody for 1000 tests.

Includes:

  • Polyclonal immunoglobulins 1546-H/IVIA anti R. solanacearum for coating,
  • Positive control,
  • Negative control,
  • Specific monoclonal antibody 8B-IVIA against R. solanacearum,
  • Anti-mouse immunoglobulins alkaline phosphatase conjugated,
  • Semi-selective medium for enrichment, vii) 10 ELISA plates, viii) Detailed protocol.

Ref: IF-Rs.

Indirect immunofluorescence (polyclonal antibodies 1546-H/IVIA), 0,5 ml.

Includes:

  • Polyclonal antibodies 1546-H/IVIA, 0,5 ml,
  • Antibodies anti-rabbit (GAR) linked with FITC, 0,5 ml.

Xanthomonas citri subsp. citri (Citrus canker)

Ref: Xcc/100.

Complete real-time PCR kit for 100 tests.

Includes:

  • 2 vials complete Master mix (universal primers+Taqman probe) for 50 reactions each,
  • 1 vial containing sterile distilled water DN/RNase-free for master mix preparation,
  • 1 tube 12mL distilled water DN/RNase-free for sample preparation,
  • 2 vials containing positive control immobilized on paper,
  • 2 vials containing healthy control immobilized on paper,
  • Detailed protocol.

Ref: Xccmix.

Complete Master mix for 50 tests.

‘Xylella fastidiosa (any subspecies)

Ref: Xf/100.

Complete kit of real-time PCR based on Harper et al. (2010, 2013) and EPPO (2016) for screening, 100 tests.
 Includes:

  • 2 vials containing lyophilized complete master mix (universal primers+Taqman FAM/TAMRA probe, according to Harper et al. (2010, 2013) for X. fastidiosa amplification (each tube contains enough master mix for 50 reactions using 9 microliters/reaction).
  • 2 vials containing distilled sterile wateer DN/RNases free for master mix and controls preparation.
  • 2 vials containing one immobilized avirulent positive control spotted on a disk of membrane (3 microliters chimeric DNA from the bacterium).
  • 2 viales containing one immobilized negative control spotted on a disk of membrane (3 microliters per spot of distilled sterile water DN/RNases free).
  • Detailed protocol.

Ref: Xfmix.

Complete master mix for 50 tests (according to Harper et al. (2010, 2013), 50 tests.
 Include: 

  • 1 vial containing lyophilized complete master mix (universal primers+Taqman FAM/TAMRA probe), according to Harper et al. (2010, 2013) for X. fastidiosa amplification, 50 reactions.

Ref: Xf-PC.

Positive control of Xylella fastiosa.
Include:

  • 1 vial containing one immobilized avirulent positive control spotted on a disk of membrane (3 microliters chimeric DNA from the bacterium).

Ref: Xf-HC.

Healthy control of Xf.
Include:

  • 1 vial containing a healthy control (3 microliters per spot of distilled sterile water DN/RNases free) immobilized on a disk of membrane

IMG_4343

Xylophilus ampelinus (Grapevine bacterial necrosis)

Ref: Xa.

DASI-ELISA complete kit based on 8C+7F/IVIA specific monoclonal antibodies for 1000 tests.

Includes:

  • Polyclonal immunoglobulins anti X. ampelinus for coating,
  • Positive control,
  • Negative control,
  • Specific monoclonal antibodies 8C+7F-IVIA against X. ampelinus,
  • Anti-mouse immunoglobulins alkaline phosphatase conjugated,
  • Detailed protocol.

Ref: IF-Xa.

Indirect immunofluorescence (specific monoclonal antibodies 8C+7F-IVIA) 0,5 ml.

Includes:

  • Specific monoclonal antibodies 8C+7F-IVIA, 0,5 ml,
  • Antibodies anti-mouse (GAM) linked with FITC, 0,5 ml.

Note

Please, contact us for any other format or amount of reagents, for bulk purchasing or for reagents against non-listed pathogens. Other products for bacteria and phytoplasmas detection are under evaluation and will be soon available.