1 package containing 2 paper membranes (7x13cm) (Whatman 3MM) for direct analysis of non-previously extracted or new samples if necessary.
3 vials (blue cap) containing 1mL distilled sterile water DN/RNases free for master mix preparation.
3 vials (yellow cap) containing lyophilized complete master mix (specific primers against ‘Ca. L. africanus, americanus, and asiaticus’ primers and TaqMan FAM/TAMRA probe) for specific detection-identification of any of the three ‘Ca. Liberibacter’ species associated to citrus HLB disease (each tube contains enough for 50 reactions using 9 μL/reaction).
3 vials (red cap) containing 1 immobilized non-virulent positive control spotted on a piece of membrane (‘Ca. Liberibacter africanus, americanus, and asiaticus’ in different vials).
3 vials (green cap) containing 1 immobilized negative citrus control/vial spotted on a piece of membrane (3 μL per spot of crude extract from a certified sweet orange healthy plant).