Viruses and viroids

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Citrus tristeza virus (CTV). Universal detection of all CTV isolates and identification of CTV-aggressives.

Ref: CTV-5.

Complete Tissue print-ELISA/Immunoprinting-ELISA® kit (3DF1+3CA5 monoclonal antibodies) for universal CTV detection, 5000 tests.

Includes:

  • 10 nitrocellulose membranes (7×13 cm) with pre-printed positive and negative citrus tissue controls,
  • Bovine sero albumin (BSA) as blocking agent,
  • CTV-specific monoclonal antibodies 3DF1+3CA5 alkaline phosphatase conjugated,
  • PBS buffer x10 concentrated,
  • Mineral salts and Tween-20 for washing buffer preparation,
  • Sigma-Fast tablets of precipitin substrate (BCIP+NBT) for alkaline phosphatase,
  • Detailed protocol.

Ref: CTV-1.

Complete Tissue print-ELISA/Immunoprinting-ELISA® kit (3DF1+3CA5 monoclonal antibodies) for universal CTV detection, 1000 tests.
  • Includes the same than CTV-5 but only 2 nitrocellulose membranes (7×13 cm) with pre-printed positive and negative citrus tissue controls.

Ref: CTV-MCA13.

Complete Tissue print-ELISA/Immunoprinting-ELISA® kit (MCA13 monoclonal antibody) for identification of aggressive CTV isolates, 1000 tests.

Includes:

  • Two nitrocellulose membranes (7×13 cm) with pre-printed with aggressive CTV positive and negative citrus tissue controls,
  • Bovine sero albumin (BSA) as blocking agent,
  • CTV-specific monoclonal antibody MCA13-alkaline phosphatase conjugated,
  • PBS buffer x10 concentrated,
  • Mineral salts and Tween-20 for washing buffer preparation,
  • Sigma-Fast tablets of precipitin substrate (BCIP+NBT) for alkaline phosphatase,
  • Detailed protocol.

Ref: CTVMem.

Box of 2 nitrocellulose membranes (7x13 cm) with pre-printed CTV-positive and CTV-healthy citrus tissue controls.

Ref: MagicDAS-CTV.

MagicDAS-ELISA kit (3DF1+3CA5 monoclonal antibodies) for universal CTV detection,1000 tests using 200 microliters/test.

Includes:

  • Rabbit polyclonal antibodies CTV-specific for coating,
  • CTV-specific monoclonal antibodies 3DF1+3CA5 alkaline phosphatase conjugated,
  • Detailed protocol.

Ref: CTVMAb-Alk.Phos.

CTV-specific monoclonal antibodies 3DF1+3CA5 alkaline phosphatase conjugated for 1000 tests using 200 microliters/test.

Ref: CTV-PC.

CTV-positive lyophilized citrus tissues control.

Ref: CTV-NC.

CTV-negative lyophilized healthy citrus tissues control.

A CTVA MagicDAS-aggressiveCTV.

Citrus viroids: Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd).

Ref: CEVd+HSVd/100.

Real-time duplex RT-PCR kit® for direct screening of CEVd and HSVd for 100 tests.

Includes:

  • 2 vials with specific primers+Taqman FAM/TAMRA probes, without RT-PCR master mix, for 50 reactions each,
  • 1 package containing 2 nylon positively charged membranes 7×13 cm,
  • 1 tube containing 12mL DN/RNase-free glycine buffer for sample preparation,
  • 2 vials containing positive CEVd and HSVd controls immobilized on paper,
  • 2 vials containing negative control immobilized on paper,
  • Detailed protocol.

Ref: CEVd+HSVd-PC.

Positive controls CEVd and HSVd for real-time RT-PCR.

Includes:

  • 2 vials with tissue-print of citrus infected material of each viroid on pieces of nylon membrane,
  • Detailed protocol of extraction.

Ref: CEVd+HSVd-NC.

Negative CEVd and HSVd controls for real-time RT-PCR.

Includes:

  • 2 vials with tissue-print of healthy citrus material on pieces of nylon membrane,
  • Detailed protocol of extraction.

A Citrus exocortis

Peach latent mosaic viroid (PLMVd).

Ref: PLMVd/100.

Real-time RT-PCR kit® for direct screening of PLMVd for 100 tests.

Includes:

  • 2 vials with specific primers+Taqman FAM/TAMRA probe, without RT-PCR master mix, for 50 reactions each,
  • 1 package containing 2 nylon positively charged membranes 7×13 cm,
  • 1 tube containing 12mL DN/RNase-free distilled water for sample preparation,
  • 2 vials containing positive PLMVd controls immobilized on paper,
  • 2 vials containing negative control immobilized on paper,
  • Detailed protocol.

Ref: PLMVd-PC.

Positive controls PLMVd for real-time RT-PCR.

Includes:

  • 2 vials with tissue-print of plant tissues PLMVd infected immobilized on pieces of nylon membrane,
  • Detailed protocol of extraction.

Ref: PLMVd-NC.

Negative controls PLMVd for real-time RT-PCR.

Includes:

  • 2 vials with tissue-print of plant tissues PLMVd-free immobilized on pieces of nylon membrane,
  • Detailed protocol of extraction

Pelargonium flower break virus (PFBV).

Ref: PFBV.

Complete Tissue print-ELISA / Immunoprinting-ELISA kit for 2000 tests.

Includes:

  • 10 nitrocellulose membranes (7×13 cm) with pre-printed positive and negative controls,
  • Bovine sero albumin (BSA) as blocking agent,
  • PFBV-specific polyclonal antibodies alkaline phosphatase conjugated,
  • PBS buffer x10 concentrated,
  • Mineral salts and Tween-20 for washing buffer preparation,
  • Sigma-Fast tablets of precipitin substrate (BCIP+NBT) for alkaline phosphatase,
  • Detailed protocol.

Plum pox virus (PPV) or Sharka virus. Universal detection of all described types.

Ref: MagicDAS-PPV.

MagicDAS-ELISA kit® “Extra”(based on 5B-IVIA/AMR monoclonal antibody) for 1000 tests using 200 microliters/test.

Includes:

  • Rabbit polyclonal antibodies R3-AMR PPV-specific for coating,
  • Cocktail of 5B-IVIA/AMR specific monoclonal antibody and PPV-specific antibodies alkaline phosphatase conjugated, for detection of any PPV-type (PPV-D, -M, -AM, -Rec, -EA, -W, -T, -C, and -CR),
  • Detailed protocol.

Ref: DASI-PPV.

DASI-ELISA kit® ( based on universal 5B-IVIA/AMR monoclonal antibody) for 1000 tests using 200 microliters/test.

Includes:

  • Rabbit polyclonal antibodies R3-AMR PPV-specific for coating,
  • 5B-IVIA/AMR specific monoclonal antibody,
  • Goat anti-mouse immunoglobulins alkaline phosphatase conjugated,
  • Detailed protocol.

Ref: 5B-PPV.

PPV-specific monoclonal antibody 5B-IVIA/AMR® for 1000 tests using 200 microliters/test.

Ref: PAb-PPV.

PPV-specific polyclonal antibodies R3-AMR (1 mg/mL purified immunoglobulins)

Ref: PPV-PC.

PPV positive lyophilized control.

Ref: PPV-NC.

PPV negative lyophilized control.

A DASI-PPV

Plum pox virus-Dideron (PPV-D). Typing test.

Ref: MagicDAS-PPV-D.

MagicDAS-ELISA kit (based on 4DG5 monoclonal antibody) for 1000 tests using 200 microliters/test.

Includes:

  • Rabbit polyclonal antibodies R3-AMR PPV-specific for coating,
  • 4DG5 PPV-D specific monoclonal antibody alkaline phosphatase conjugated, for specific detection of the majority of PPV-D isolates,
  • Detailed protocol.

Ref: DASI-PPV-D.

DASI-ELISA kit® ( based on PPV-D specific 4DG5 monoclonal antibody) for 1000 tests using 200 microliters/test.

Includes:

  • Rabbit polyclonal antibodies R3-AMR PPV-specific for coating,
  • 4DG5 PPV-D specific monoclonal antibody,
  • Goat anti-mouse immunoglobulins alkaline phosphatase conjugated,
  • Detailed protocol.

Ref: 4D-PPV-D.

PPV-D specific monoclonal antibody 4DG5 for 1000 tests using 200 microliters/test.

Ref: PPV-D-PC

PPV-D positive lyophilized control.

Plum pox virus-Marcus (PPV-M). Typing test.

Ref: MagicDAS-PPV-M.

MagicDAS-ELISA kit (based on AL monoclonal antibody) for 1000 tests using 200 microliters/test.

Includes:

  • Rabbit polyclonal antibodies PPV-specific for coating,
  • AL PPV-M specific monoclonal antibody alkaline phosphatase conjugated, for identification of PPV-M, -Rec, -T and –AM isolates,
  • Detailed protocol.

Ref: DASI-PPV-M.

DASI-ELISA kit® (based on AL PPV-M, specific monoclonal antibody) for 1000 tests using 200 microliters/test.

Includes:

  • Rabbit polyclonal antibodies R3-AMR PPV-specific for coating,
  • AL PPV-M, -Rec, -T and –AM specific monoclonal antibody,
  • Goat anti-mouse immunoglobulins alkaline phosphatase conjugated,
  • Detailed protocol.

Ref: AL-PPV-M.

PPV-M, -Rec, -T and -AM specific monoclonal antibody AL for 1000 tests using 200 microliters/test.

Ref: PPV-M-PC

PPV-M positive lyophilized control.

Kit DAS-ELISA PPV

Plum pox virus-Cherry (PPV-C). Typing test.

Ref: MagicDAS-PPV-C.

MagicDAS-ELISA kit (based on C monoclonal antibody) for 1000 tests using 200 microliters/test.

Includes:

  • Rabbit polyclonal antibodies R3-AMR PPV-specific for coating,
  • C PPV-Cherry specific monoclonal antibody alkaline phosphatase conjugated, for specific detection of PPV-C isolates adapted to cherry,
  • Detailed protocol.

Ref: DASI-PPV-C.

DASI-ELISA kit® (based on C PPV-C specific monoclonal antibody) for 1000 tests using 200 microliters/test.

Includes:

  • Rabbit polyclonal antibodies R3-AMR PPV-specific for coating,
  • C PPV-Cherry specific monoclonal antibody,
  • Goat anti-mouse immunoglobulins alkaline phosphatase conjugated,
  • Detailed protocol.

Ref: C-PPV-C.

PPV-Cherry specific monoclonal antibody C for 1000 tests using 200 microliters/test.

Ref: PPV-C-PC.

PPV-C positive lyophilized control.

Potato spindle tuber viroid (PSTVd).

Ref: PSTVd/100.

Real-time RT-PCR kit® for direct screening of PSTVd for 100 tests.

Includes:

  • 2 vials with specific primers+Taqman FAM/TAMRA probe, without RT-PCR master mix, for 50 reactions each,
  • 1 package containing 2 nylon positively charged membranes 7×13 cm,
  • 1 tube containing 12mL DN/RNase-free distilled water for sample preparation,
  • 2 vials containing positive PSTVd controls immobilized on paper,
  • 2 vials containing negative control immobilized on paper,
  • Detailed protocol.

Ref: PSTVd-PC.

Positive control PSTVd for real-time RT-PCR.

Includes:

  • 2 vials with 1 tissue-print of plant tissues PSTVd infected immobilized on pieces of nylon membrane,
  • Detailed protocol of extraction.

Potato virus X (PVX).

Ref: MagicDAS-PVX.

MagicDAS-ELISA kit® (based on 3CB9/INGENASA PVX specific monoclonal antibodies) for 1000 tests using 200 microliters/test.

Includes:

  • Monoclonal antibodies PVX-specific for coating,
  • Monoclonal antibodies PVX-specific alkaline phosphatase conjugated,
  • Detailed protocol.

Potato virus Y (PVY).

Ref: MagicDAS-PVY.

MagicDAS-ELISA kit® (based on 1E10+10E3/INGENASA PVY specific monoclonal antibodies) for 1000 tests using 200 microliters/test.

Includes:

  • Monoclonal antibodies PVY-specific for coating,
  • Monoclonal antibodies PVY-specific alkaline phosphatase conjugated,
  • Detailed protocol.

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Tomato spotted wilt virus (TSWV).

Ref: TSWV.

Complete Tissue print-ELISA / Immunoprinting-ELISA kit for 2000 tests.

Includes:

  • 10 nitrocellulose membranes (7×13 cm) with pre-printed positive and negative controls,
  • Bovine sero albumin (BSA) as blocking agent,
  • TSWV-specific polyclonal antibodies alkaline phosphatase conjugated,
  • PBS buffer x10 concentrated,
  • Mineral salts and Tween-20 for washing buffer preparation,
  • Sigma-Fast tablets of precipitin substrate (BCIP+NBT) for alkaline phosphatase,
  • Detailed protocol.

Tomato yellow leaf curl virus (TYLCV).

Ref: TYLCV.

Complete Tissue print-ELISA / Immunoprinting-ELISA kit for 2000 tests.

Includes:

  • 10 nitrocellulose membranes (7×13 cm) with pre-printed positive and negative controls,
  • Bovine sero albumin (BSA) as blocking agent,
  • TYLCV-specific polyclonal antibodies alkaline phosphatase conjugated,
  • PBS buffer x10 concentrated,
  • Mineral salts and Tween-20 for washing buffer preparation,
  • Sigma-Fast tablets of precipitin substrate (BCIP+NBT) for alkaline phosphatase,
  • Detailed protocol.

Note

Please, contact us for any other format or amount of reagents or for bulk purchasing. We offer a special discount when ordering 10 or more units of the same or different PLANT PRINT products.